| Association | : |  Federação de Futebol da Guiné-Bissau |
| Nickname(s) | : | Djurtus |
| Confederation | : | CAF - WAFU-UFOA |
| FIFA code | : | GNB |
| FIFA rank | : | 118 (12 February 2024) |
| Most Caps | : | Jonas Mendes (56) |
| Top Scorer | : | Nando Có (9) |
| Home stadium | : | Estádio 24 de Setembro, Estádio Lino Correia |
| Head coach | : | Baciro Candé |
Guinea-Bissau - Africa Cup of Nations 2023
Guinea-Bissau's 39-man provisional list was announced by CAF on 20 December 2023. The final squad of 27 players was announced on 5 January 2024. Goalkeeper Celton Biai has declined the invitation as he wanted to focus on his club. Caps and goals correct as of 22 January 2024, after the match against Nigeria.
| No | Position | Player Name | Date of birth (age) | Caps | Goals | Club |
|---|---|---|---|---|---|---|
| GK | Jonas Mendes (captain) | 20-11-1989 (34) | 58 | 0 |  Kalamata | |
| GK | Ouparine Djoco | 22-04-1998 (25) | 5 | 0 |  Francs Borains | |
| GK | Fernando Embadje | 06-07-2003 (20) | 0 | 0 |  Alcanenense | |
| DF | Sori Mané | 03-04-1996 (27) | 33 | 0 | Moreirense | |
| DF | Nanú | 17-05-1994 (29) | 26 | 0 |  Samsunspor | |
| DF | Opa Sanganté | 01-02-1991 (33) | 24 | 0 |  Dunkerque | |
| DF | Fali Candé | 24-01-1998 (26) | 23 | 0 | Metz | |
| DF | Marcelo Djaló | 08-10-1993 (30) | 19 | 1 |  Hércules | |
| DF | Jefferson Encada | 17-04-1998 (25) | 19 | 0 |  Pharco | |
| DF | Houboulang Mendes | 04-05-1998 (25) | 4 | 0 | Almeria | |
| DF | Edgar Ié | 01-05-1994 (29) | 3 | 0 | İstanbul Başakşehir | |
| MF | Moreto Cassamá | 16-02-1998 (25) | 24 | 0 |  Omonia | |
| MF | Alfa Semedo | 30-08-1997 (26) | 24 | 2 |  Al-Tai | |
| MF | Dálcio | 22-05-1996 (27) | 17 | 0 | APOEL | |
| MF | Mauro Rodrigues | 15-04-2001 (22) | 14 | 2 |  Yverdon-Sport | |
| MF | Janio Bikel | 28-06-1995 (28) | 13 | 0 | Gaziantep | |
| MF | Carlos Mané | 11-03-1994 (29) | 5 | 1 | Kayserispor | |
| MF | Famana Quizera | 25-04-2002 (21) | 4 | 0 | Vizeu | |
| MF | Nito Gomes | 03-02-2002 (22) | 3 | 1 | Marítimo | |
| MF | Zidane Banjaqui | 15-12-1998 (25) | 1 | 1 | Feirense | |
| FW | Mama Baldé | 06-11-1995 (28) | 25 | 4 | Lyon | |
| FW | Zinho Gano | 13-10-1993 (30) | 7 | 4 | Zulte Waregem | |
| FW | Franculino Djú | 28-06-2004 (19) | 6 | 1 |  Midtjylland | |
| FW | Zé Turbo | 22-10-1996 (27) | 6 | 1 |  Pari NN | |
| FW | Carlos Mendes Gomes | 14-11-1998 (25) | 5 | 0 |  Bolton Wanderers | |
| FW | Marciano Sanca | 06-11-2004 (19) | 3 | 0 | Almeria B | |